A Double-Deck Self-Digitization Microfluidic Chip for Digital PCR
In this work, a double-deck microfluidic chip was presented for digital PCR application.This chip consists of two reverse-placed micro-patterned polydimethylsiloxane (PDMS) layers Dab Inserts between the top and bottom glass substrates.Each micropatterned PDMS layer contains more than 20,000 cylindrical micro-chambers to hold the partitioned drople